Composite

Part:BBa_I52000:Design

Designed by: Reshma Shetty   Group:   (2006-03-13)

ccdB and minimal pUC19 derived high copy origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a composite part of BBa_P1011 and BBa_I50020. It is designed to be placed in the multiple cloning site of BioBricks vectors. Vectors with this part in the multiple cloning site will be selected against during BioBricks assembly because BBa_P1011 is lethal to most cell strains. Also, BBa_I50020 enables the vector to be propagated at high copy regardless of the other origins on the vector.

Note that in the Registry, this part is assembled via standard BioBricks assembly. However, in reality we are planning to synthesize this part and therefore it may not have any scar.


Source

References